The prodigious advantages of in vitro aseptic technique of clonal propagation i. Recent studies2 4 revealed that most chlorophyllous plants in vitro have the ability to grow photoautotrophically, and that the low co 2 concentration in the airtight. This in vitro technique helps to raise pathogen free plant and to maintain them. Pdf in vitro micropropagation of disease free rose rosa indica l. Micropropagation can be used to produce disease free plants. Alstroemeria, cytokinins, agar, tissue culture and micropropagation.
Although the tissue culture propagation is widely disseminated for medicinal. An efficient method for in vitro propagation of alstroemeria. Genetically reproducing plants in vitro can cause the plants to become less resistant to diseases and infections. S2 micropropagation and in vitro techniques 2nd international symposium. Techniques of invitro micro propagation tissue culture. Micro propagation is very useful in case of dioecious plants, because there the seed progeny yield is 50% male and 50% female, but this technique helps to get the progeny according to the desired sex. In vitro tissue culture of apple and other malus species. Pdf a rapid protocol for efficient shoot organogenesis and plant. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. In vitro tissue culture for apple breeding and biotechnology. Micropropagation stages, types, applications, advantages. The main advantage of micropropagation is the production of many plants that are clones of each other. Starting from in vitro culturing of apical and nodal segment explants, 0.
A method has been developed for the rapid in vitro micropropagation of the pri mocanefruiting. The technique has been applied to a substantial number of commercial vegetatively propagated plant species. In vitro propagation and acclimatization of lippia. In vitro plant regeneration and micropropagation of liriope platyphylla. An efficient micropropagation protocol for an endangered. Micro propagation has significant uses in vegetative propagation of horticulturally important species and cultivars.
Pdf the rose is the most popular ornamental plant in the world, as well as the most important cut flower. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. The best results were obtained in the treatments containing. After 4 wk of in vitro culture, rhizomes grown in a free.
The cultivation and growing of orchids and other plants in vitro is an extremely important asset to the. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of disease free plants and its merits and demerits. Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. Micropropagation has a number of advantages over traditional plant propagation techniques. Pdf in vitro plant regeneration and micropropagation of liriope. Project on micropropagation free download as powerpoint presentation. Establishment of an in vitro micropropagation protocol for.1329 718 339 438 1298 234 532 270 575 1390 152 829 214 1460 1064 253 610 439 444 245 190 93 504 539 579 279 1125 1142 1288 1163 147 527 264 1278 876